Thymosin Beta-4 Dosage & Reconstitution Guide
How it differs from TB-500, reconstitution math, and syringe draw reference — everything in one place.
What Is Thymosin Beta-4?
Thymosin Beta-4 (Tβ4) is a naturally occurring 43-amino acid peptide found throughout human and animal tissues, playing a role in cellular structure via actin regulation. It has been investigated extensively in published preclinical and clinical research literature for its potential roles in wound healing, tissue repair, and anti-inflammatory processes. Thymosin Beta-4 is the full-length naturally occurring peptide, distinct from TB-500, which is a shorter synthetic peptide modeled after its active region.
Thymosin Beta-4 and TB-500 are related but distinct compounds. Most of the foundational published research — including wound healing and cardiac tissue studies — was conducted using full-length Thymosin Beta-4, not the synthetic fragment TB-500 that is commonly sold in the research peptide market. This is a frequently misunderstood distinction in the research community.
Thymosin Beta-4 vs TB-500
Understanding the Difference
| Parameter | Thymosin Beta-4 | TB-500 |
|---|---|---|
| Structure | Full-length, 43 amino acids | Synthetic fragment, 4 amino acids (active region) |
| Origin | Naturally occurring in tissue | Synthetic, modeled after Tβ4's active region |
| Research volume | Larger body of published literature | Smaller, more commercially focused research base |
| Availability | Less commonly sold by research vendors | Most common form sold in the research peptide market |
| Cost | Typically higher per mg | Typically lower per mg |
Because TB-500 is modeled after the active region of Thymosin Beta-4, research conducted on TB-500 specifically is more limited than the body of literature on the full-length peptide. Researchers should be aware of which compound a given study or vendor product actually refers to.
Common Research Doses
| Research application | Dose range | Frequency |
|---|---|---|
| General research | 2–4 mg | Once or twice weekly |
| Loading phase research | 4–6 mg | Twice weekly for 4–6 weeks |
Dosing patterns for full-length Thymosin Beta-4 are commonly discussed using similar ranges to TB-500, given their related mechanisms, though they are not identical compounds.
Reconstitution Reference
| Vial size | BAC water | Concentration | Doses at 2 mg | Doses at 4 mg |
|---|---|---|---|---|
| 5 mg | 1.0 mL | 5.0 mg/mL | 2 | 1 |
| 10 mg | 2.0 mL | 5.0 mg/mL | 5 | 2 |
| 10 mg | 1.0 mL | 10.0 mg/mL | 5 | 2 |
Calculate your exact syringe draw
Open the Calculator →Syringe Draw Reference
| Dose | Draw to |
|---|---|
| 2 mg | 40 units |
| 4 mg | 80 units |
| 6 mg | 120 units * |
* Volumes above 100 units (1 mL) require multiple injections or a higher concentration.
Sourcing
Recommended Supplier
PSPeptides carries third-party tested, research-grade peptides with published COAs. Confirm with the supplier whether a product is full-length Thymosin Beta-4 or the TB-500 fragment before purchasing, as the two are frequently confused in vendor listings.
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