TB-500 Dosage & Protocol Guide
Loading vs maintenance dosing, reconstitution math, syringe draw reference, and the TB-500 + BPC-157 stack — everything in one place.
What Is TB-500?
TB-500 is a synthetic peptide modeled after the active region of Thymosin Beta-4 (Tβ4), a naturally occurring peptide found in many human and animal tissues. It has attracted research interest particularly in the context of musculoskeletal and connective tissue studies. TB-500 is not approved by the FDA for human use and is classified as a research chemical.
TB-500 Dosing Protocols
Loading vs Maintenance Phase
Most TB-500 research protocols use a front-loaded approach — a higher-frequency loading phase for the first 4–6 weeks, followed by a reduced maintenance phase. The rationale is to achieve higher early exposure before transitioning to a lower-frequency protocol.
| Protocol type | Dose range | Frequency | Duration |
|---|---|---|---|
| Loading phase | 4–6 mg | Twice weekly | 4–6 weeks |
| Maintenance phase | 2–3 mg | Once weekly | Variable duration |
| Acute focus | 5–6 mg | Twice weekly | 4–8 weeks |
| Lower range | 2 mg | Once weekly | Variable duration |
TB-500 is dosed in milligrams (mg), not micrograms. This distinguishes it from BPC-157 which is dosed in mcg. Always confirm your units before calculating.
Reconstitution Reference
How Much BAC Water to Add
| Vial size | BAC water | Concentration | Doses at 2 mg | Doses at 5 mg |
|---|---|---|---|---|
| 5 mg | 1.0 mL | 5.0 mg/mL | 2 | 1 |
| 10 mg | 2.0 mL | 5.0 mg/mL | 5 | 2 |
| 10 mg | 4.0 mL | 2.5 mg/mL | 5 | 2 |
| 20 mg | 4.0 mL | 5.0 mg/mL | 10 | 4 |
Calculate your exact syringe draw
Open the Calculator →Syringe Draw Reference
| Dose | Draw to |
|---|---|
| 1 mg | 20 units |
| 2 mg | 40 units |
| 3 mg | 60 units |
| 4 mg | 80 units |
| 5 mg | 100 units |
| Dose | Draw to |
|---|---|
| 1 mg | 40 units |
| 2 mg | 80 units |
| 2.5 mg | 100 units |
| 3 mg | 120 units * |
| 5 mg | 200 units * |
* Volumes above 100 units (1 mL) require multiple injections. Reconstitute at 5.0 mg/mL for doses of 3 mg or above.
TB-500 + BPC-157 Stack
The Most Common TB-500 Stack
TB-500 and BPC-157 are frequently used together in research protocols. TB-500 is modeled after the active region of Thymosin Beta-4 and has been investigated in preclinical research for potential effects related to cell migration, angiogenesis, inflammatory regulation, and tissue remodeling. BPC-157 has been investigated for potential effects related to angiogenesis and tissue repair processes in preclinical models. The combination is popular in the research community based on the hypothesis that their proposed mechanisms may be complementary.
| Compound | Dose | Frequency |
|---|---|---|
| TB-500 | 2–5 mg | Once or twice weekly |
| BPC-157 | 250–500 mcg | Once or twice daily |
TB-500 and BPC-157 can be drawn into the same syringe for a combined injection or administered separately. Combining compounds in the same syringe has been described in some research communities but introduces additional variables. Calculate each compound's draw volume separately using the calculator before combining.
Sourcing
Recommended Research Supplier
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The TB-500 Protocol Guide
The complete 10-section research protocol — loading vs maintenance dosing, reconstitution walkthrough, syringe draw reference tables, BPC-157 stack guide with mixing instructions, storage, and the 6 most common mistakes.