SS-31 (Elamipretide): Dosage, Calculator & Reconstitution Guide
A mitochondria-targeted research peptide studied for its interaction with cardiolipin in the inner mitochondrial membrane — reconstitution math, dosing ranges, and calculator, for research context only.
What Is SS-31?
SS-31, also known as elamipretide, is a synthetic tetrapeptide (D-Arg-2′,6′-dimethyltyrosine-Lys-Phe-NH2) developed as a mitochondria-targeted agent. Its proposed mechanism involves selective binding to cardiolipin, a phospholipid concentrated in the inner mitochondrial membrane, which may help stabilize cristae architecture and reduce mitochondrial reactive oxygen species generation in preclinical models.
Research contexts for SS-31 include primary mitochondrial myopathy, heart failure with preserved ejection fraction, neurodegenerative models, and aging-related bioenergetic decline. Most evidence comes from cell and animal studies plus a limited set of human clinical trials; SS-31 is not approved by the FDA for human use and this page is a summary of the research literature, not a health claim.
Dosing Reference
| Typical Vial Size | BAC Water Volume | Common Dose Range | Typical Frequency |
|---|---|---|---|
| 10 mg | 2.0 mL | 1–5 mg / day | Once daily |
| 50 mg | 2.0 mL | 5–10 mg / day | Once daily |
SS-31 is dosed in milligrams per day in research protocols, not micrograms. Published clinical work has used fixed daily amounts (e.g., 40 mg/day), while research-community reports often cite 5–10 mg/day. Dosing is highly study-specific.
Reconstitution & Calculator
For a 10 mg lyophilized vial, slowly add 2 mL of bacteriostatic water down the side of the vial, swirl gently until the powder fully dissolves, and store refrigerated. That yields a 5 mg/mL working concentration — a 1 mg dose is 200 microliters (20 units on a U-100 insulin syringe), and a 5 mg dose is 1 mL (100 units). For a 50 mg vial with 2 mL BAC water the concentration is 25 mg/mL, so a 5 mg dose is 200 microliters (20 units).
Calculate your exact draw
Storage & Stability
| State | Storage | Shelf Life |
|---|---|---|
| Lyophilized (unmixed) | Refrigerate or freeze; protect from light | 12–24 months |
| Reconstituted vial | Refrigerate 2–8°C; do not freeze | ~30 days |
| Diluted or aliquoted | Refrigerate short-term; avoid freeze-thaw | Use immediately or per protocol |
SS-31 should be protected from heat, light, and repeated freeze-thaw cycles. Reconstituted solution should be clear; discard if cloudiness or particles persist after gentle mixing.
Frequently Asked Questions
Next Steps
- → Peptide Calculator for exact syringe draw math
- → Full Peptide Dosage Chart to compare across compounds
Recommended Supplier
PSPeptides carries third-party tested, research-grade peptides with published COAs.
Discount Code: BANANAS10
Shop PSPeptides →Affiliate disclosure: The Protocol Kit earns a commission from qualifying purchases at no additional cost to you.