RESEARCH PROTOCOL

Kisspeptin: Dosage, Calculator & Reconstitution Guide

A reproductive/hormonal axis research peptide studied for its role in GnRH signaling — reconstitution math, dosing ranges, and calculator, for research context only.

What Is Kisspeptin?

Kisspeptin is a family of peptide hormones encoded by the KISS1 gene, with kisspeptin-10 being the most commonly referenced fragment in research. It is produced in the hypothalamus and acts on KISS1R (formerly GPR54) receptors on GnRH neurons. Through this pathway, kisspeptin plays a central role in regulating the pulsatile release of gonadotropin-releasing hormone (GnRH), which in turn drives luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion from the pituitary.

The proposed mechanism most frequently cited in the literature is modulation of the hypothalamic-pituitary-gonadal (HPG) axis. Research has examined kisspeptin in contexts such as hypogonadotropic hypogonadism, hypothalamic amenorrhea, and fertility-related endocrine studies. Kisspeptin is not approved by the FDA for human use and this page is a summary of the research literature, not a health claim.

Dosing Reference

Typical Vial SizeBAC Water VolumeCommon Dose RangeTypical Frequency
2 mg1.0 mL100–300 mcgSingle dose or study-defined
5 mg2.0 mL100–300 mcgSingle dose or study-defined
10 mg2.0 mL100–300 mcgSingle dose or study-defined

Kisspeptin dosing in published research is highly protocol-specific and often administered as a single research dose or short infusion series. The ranges above reflect commonly reported research quantities, not clinical recommendations.

Reconstitution & Calculator

For a 5 mg lyophilized vial, slowly add 2 mL of bacteriostatic water down the side of the vial, swirl gently until the powder fully dissolves, and store refrigerated. That yields a 2.5 mg/mL working concentration — a 250 mcg dose is 100 microliters (10 units on a U-100 insulin syringe). For a more concentrated solution, use 1 mL of BAC water to reach 5 mg/mL, where the same 250 mcg dose is 50 microliters (5 units).

Storage & Stability

StateStorageShelf Life
Lyophilized (unmixed)Refrigerate or freeze; protect from light12–24 months
Reconstituted vialRefrigerate 2–8°C; do not freeze~30 days
Diluted or aliquotedRefrigerate short-term; avoid freeze-thawUse immediately or per protocol

Kisspeptin is a peptide and should be protected from heat, light, and repeated freeze-thaw cycles. Reconstituted solution should be clear; discard if cloudiness or particles persist after gentle mixing.

Frequently Asked Questions

Next Steps

Recommended Supplier

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Disclaimer: The information on this page is provided for research and educational purposes only. Kisspeptin is not approved by the FDA for human use. Nothing on this page constitutes medical advice. Consult a qualified healthcare professional before making any health-related decisions.